Observations on pathogenicity between A and B subtype of hMPV in mice

VernacularTitle:人偏肺病毒A与B基因型对小鼠致病性的观察
Author: Zhen ZHOU ; Yuxia CUI ; Yongbo ZHANG ; Xian QIN ; Rongpei LI ; Ping LIU ; Ying DOU ; Lijia WANG ; Xiaodong ZHAO ; Yao ZHAO
Publication Type:Journal Article
Keywords: Human metapneumovirus (hMPV); Genotype; Pathogenicity
From: Chinese Journal of Microbiology and Immunology 2012;32(1):36-40
CountryChina
Language:Chinese
Abstract: ObjectiveTo investigate the difference of pathogenicity between the two genotypes of human metapneumovirus(hMPV) for the further research.MethodsAt various time after hMPV infection in BALB/c mice,viral titers of lung tissue were measured by real-time RT-PCR,pathology was assessed by a histopathological scoring system,airway responsiveness was assayed by animal lung function monitoring equipment.Pathogenicity was then measured by detailed evaluation through the results above.Results There is no significant difference in weight of mice between control group and experimental group through dynamic monitoring ; though the difference was exists in airway responsiveness and pathological changes of mice between control group and experimental group,the differences were not statistically in airway reaction,pathological changes and virus drops among the three groups of experimental group.ConclusionThere is no difference in pathogenicity between the two subtypes of hMPV in infection of BALB/c mice,viral genotype do not appear to be associated with pathogenicity.