The preliminary study of CD4+ Vα9-J27/Vβ29-D1-J2 tetramers in detecting Mycobacterium tuberculosis infections
10.3760/cma.j.issn.0254-5101.2012.01.005
- VernacularTitle:应用CD4+Vα9-J27/Vβ29-D1-J2四聚体检测结核分枝杆菌感染的初步研究
- Author:
Dan XIE
;
Kouxing ZHANG
;
Xuanjing DU
;
Yimin FANG
;
Yan LI
;
Yi CHEN
;
Jianbo ZHANG
;
Ming GAO
;
Xiaomin LAI
- Publication Type:Journal Article
- Keywords:
Tuberculosis;
CD4+ TCR tetramer;
CD14+ monocytes and macrophages
- From:
Chinese Journal of Microbiology and Immunology
2012;32(1):20-24
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the specificity of CD4+ Vα9-J27/Vβ29-D1-J2 tetramer in detecting Mycobacterium tuberculosis(MTB) infections.MethodsThe above TCR tetramer by using biotinylated monomers expressed and purified from constructed stable Drosophila Schneider 2 cell( S2 cell) lines was prepared.The PE-labled TCR tetramer was used to costain with S2 cell lines expressing MTB prptide/HLA-DR complexes on the cell membrane,and also was used to detect tetramer-bound CD14+ monocytes and macrophages in the peripheral blood mononuclear cells (PBMC) of pulmonary tuberculosis (PTB) patients and three control groups by flow cytometric analysis.And the FITC-labled tetramer was used to examine tetramer-bound CD14+ monocytes and macrophages,and MTB antigen-specific and tetramer-bound cells by in situ staining.ResultsThe TCR tetramer was well binding with S2 cell lines expressing C14/HLA-DR *1504 on the cell membrane.By flow cytometric analysis,the percentage of tetramer-bound CD14+ monocytes and macrophages in PTB patients group was higher than the other three control groups( P＜0.001 ).By in situ staining,tetramer-bound CD14+ monocytes and macrophages,and MTB antigen-specific and tetramer-bound cells were positive in PTB tissue and negative in control pneumonia tissue.ConclusionThe spcificity of TCR tetramer in monitoring MTB infections by flow cytometric analysis and in situ staining could be seen,which laid a laboratory foundation in the diagnosis and immune mechanism research of TB by using TCR tetramers.
