Simultaneous detection of four types of human herpes virus by AllGlo quadruple fluorescence quantitatve PCR
10.3760/cma.j.issn.0254-5101.2012.01.018
- VernacularTitle:AllGlo探针4重荧光定量PCR技术同时检测4种疱疹病毒
- Author:
Daojun YU
;
Shenghai WU
;
Xianjun WANG
;
Wenjuan TONG
- Publication Type:Journal Article
- Keywords:
Human herpes virus (HHV);
AllGlo probe;
Quadruple fluorescence quantitative PCR;
Specificity;
Sensitivity
- From:
Chinese Journal of Microbiology and Immunology
2012;32(1):78-83
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo optimize the condition of multiple fluorescence quantitative PCR and establish a new assay of four human herpes virus (HHV) detected by AllGlo quadruple fluorescence quantitative PCR.MethodsFour HHV including HSV-1,HSV-2,EBV and CMV were identified by sequence analysing the qualitative PCR production.Furthermore,they were quantitatively detected by AllGlo and TaqMan multiple fluorescence quantitative PCR respectively.ResultsBoth the positive rate and specificity of AllGlo and TaqMan in detecting single HHV achieved 100％.And AllGlo single fluorescence quantitative PCR prevailed over TaqMan's by Ct of 1-3.Four HHV can be simultaneously detected by AllGlo quadruple fluorescence quantitative PCR,comparing to the only two by TaqMan.ConclusionAllGlo fluorescence quantitative PCR assay allows a higher throughput,sensitivity and specificity than TaqMan in detection and thus provides a board prospect.
