Neuropathological characters of delayed cortical infarcts after transient unilateral brain ischemia in Mongolian gerbils
10.3760/cma.j.issn.1006-7876.2012.02.013
- VernacularTitle:蒙古沙鼠一过性脑缺血模型迟发性脑梗死的病理学特点
- Author:
Shengjun XIAO
;
Xiaoling ZHANG
;
Fang GUO
;
Jingyan LU
;
Jing LIN
;
Qiuyue CHEN
;
Liyuan SUN
- Publication Type:Journal Article
- Keywords:
Eosinophilic neuron;
Reactive astrocyte;
Brain ischemia;
Delayed astrocytic death;
Mongolian gerbil
- From:
Chinese Journal of Neurology
2012;45(2):124-128
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the distribution of eosinphililic neurons ( ENs),reactive astrocytes ( RAs),and infarction after transient cerebal ischemia,and the time profile of pathomorphological changes.Methods Unilateral forebrain ischemia was induced in Mongolian gerbils by two 10 minutes unilateral common carotid artery occlusions with a 5 hours interval.Laser Doppler flowmetry was used to detect intra-ischemic anterior cortex blood flow.Animals were sacrificed at 24 hours,4 days,2 weeks,4 weeks,16 weeks and the brain were prepared for pathomorphological assay.Results Intra-ischemic laser Doppler flowmetry show significant ischemia during carotid artery occlusion:22.1％ ± 9.5％,26.3％ ± 4.9％,37.5％ ± 3.5％,F =67.219,P ＜ 0.01 ; the decrease was significantly greater in the anterior cortex.ENs appeared in middle and deep layers at 24 hours postischemia,and ENs area extend to all layers of cortex by 4 days.Large areas of high EN density ( ≥80/mm2) evolved to infarcts between 4 days and 4 weeks.Posterior cortex evolved to low EN area ( ＜ 80/mm2) without transformation into infarcts.RAs were consistently distributed in areas with ENs,and RA areas with high EN density were largely transformed into infarcts between 4 days and 4 weeks. Delayed astrocytic death took place in the RA areas with high EN density.Conclusion Density of ENs is an important indicator of delayed astrocytic death and infarction in postischemic tissue.
