Effects of all-trans retinoic acid, acitretin and tazarotene on apoptosis and active caspase-3 in human melanoma cells A375
10.3760/cma.j.issn.1671-0290.2012.01.018
- VernacularTitle:全反式维A酸、阿维A和他扎罗汀对人黑素瘤细胞A375凋亡及活性Caspase-3的影响及意义
- Author:
Liwei RAN
;
Dong LAN
;
Hongmei LIU
- Publication Type:Journal Article
- Keywords:
Retinoids, Melanoma cell;
Apoptosis
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2012;18(1):60-63
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of all -trans retinoic acid (ATRA),acitretin and tazarotene on apoptosis and active caspase-3 of human melanoma cells A375 and to discuss the relevant significance.Methods The effects of retinoids on apoptosis and active caspase-3 of A375 cells were examined in vitro.Early apoptosis analysis by double staining with annexin V-FITC and PI,and active caspase 3 analysis by the staining of FITC conjugated monoclonal rabbit anti active caspase-3 antibody were measured by flow cytometer.Results ATRA and acitretin induced apoptosis of A375 cells (both P＜0.05),but tazarotene was not effective (P＞0.05).ATRA and acitretin elevated the cell population with detectable active caspase-3 (both P＜0.05),but tazarotene was not effective either (P＞0.05).Acitretin played a most prominent role among the retinoids. Early apoptosis ratio was significantly and positively correlated with active caspase-3 percentage in both ATRA and acitretin (P＜0.05).Conclusions Acitretin and ATRA induce apoptosis of A375 cells in which caspase is critical.Retinoids are prospectively applied as an assisting alternative for treating melanoma.
