Study on the role of axon guidance factor Semaphorin 5A in the invasion and metastasis of human gastric cancer
10.3760/cma.j.issn.0254-1432.2012.02.008
- VernacularTitle:神经导向分子Semaphorin5A在人胃癌侵袭和转移过程中作用的研究
- Author:
Guoqing PAN
;
Xiangling ZHANG
;
Junyu REN
;
Jianbo LU
;
Hongmei FU
- Publication Type:Journal Article
- Keywords:
Semaphorins;
Stomach neoplasms;
Gene expression;
RNA interference;
Neoplasm invasiveness;
Neoplasm metastasis;
Cell adhesion
- From:
Chinese Journal of Digestion
2012;32(2):103-107
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the correlation between axon guidance factor Semaphorin 5A and clinicopathological features and its role in the invasion and metastasis of gastric cancer.Methods The expression of Semaphorin 5A in gastric cancer tissues of 171 patients with different gender,age,histological type and TNM stage was detected with immunohistochemistry assay.The expression of Semaphorin 5A was determined by Western blotting assay in gastric cancer cell lines SGC7901 and MKN-45 with metastatic ability and gastric cancer cell lines SNU-1 and AGS without metastatic ability.With RNA interfere technique(RNAi),Semaphorin 5A siRNA expression vector was constructed and transfected into gastric cancer cell line SGC7901.The stable gastric cancer cell line down-expressing Semaphorin 5A was established.The effect of Semaphorin 5A gene silencing on the adhesion,migration and invasion of gastric cancer cell was examined by cell adhesion test,wound healing test and transwell chamber assays.Results The expression level of Semaphorin 5A was correlated with the differentiation degree of gastric cancer(x2 =6.32,P =0.01),lymphnode metastasis(x2 =7.68,P=0.01)and distant metastasis of gastric cancer(x2 =13.67,P =0.00),not correlated with age(x2 =0.21,P=0.79),gender(x2=1.79,P=0.15)and the depth of gastric cancer invasion(x2=1.34,P=0.55).The expression of Semaphorin 5A in cell lines SGC7901 and MKN-45 was significantly higher than that of cell lines SNU-1 and AGS(P＜0.01).Semaphorin 5A gene silencing significantly suppressed the adhesion,migration and invasion abilities of gastric cancer cells.Conclusion Semaphorin 5A may play a catalytic role in the invasion and metastasis of gastric cancer through increasing the adhesion,migration and invasion abilities of gastric cancer cell.
