The regulation of BLyS expression levels through MAPK signal pathway in multiple myeloma
10.3760/cma.j.issn.0254-5101.2011.12.007
- VernacularTitle:多发性骨髓瘤中MAPK信号通路对BLyS表达的调控研究
- Author:
Guang XU
;
Jiang PU
;
Shaopeng CHU
;
Xudong WANG
;
Xianjuan SHEN
;
Xinhua WU
;
Xia ZHANG
;
Changjiang SUN
;
Shaoqing JU
- Publication Type:Journal Article
- Keywords:
Multiple myeloma;
B-lymphocyte stimulator (BLyS);
MAPK signal pathway
- From:
Chinese Journal of Microbiology and Immunology
2011;31(12):1081-1086
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the activation of MAPK signal pathway in multiple myeloma and the regulation of BLyS expression levels through MAPK signal pathway; preliminarily study the role of MAPK signal pathway in the up-regulation of BLyS expression levels induced by IFN-γ.MethodsActivated MAPK pathway were detected by Western blot,while the expression of BLyS were detected with RT-PCR and Western blot,and Western blot investigated the effect of MAPK pathway on BLyS expression levels induced by IFN-γ.ResultsIn addition to the expression of ERK,JNK,p38,p-JNK was also expressed in MM cell lines,the MAPK pathway inhibitor targeting JNK SP600125 can down-regulate the expression of BLyS,and its activator anisomycin can up-regulate the expression of BLyS.SP600125 restrained the proliferation and survival of MM cells.ConclusionJNK/SAPK pathway was activated in MM cells; The activated degree of JNK/SAPK pathway and the expression level of BLyS was positively correlated.JNK/SAPK pathway play an important role in the up-regulation of BLyS expression levels induced by IFN-γ.
