Molecular characteristic of dominant serotypes of Vibrio parahaemolyticus isolated from foodborne disease outbreaks in Guangdong province
10.3760/cma.j.issn.0254-5101.2011.12.009
- VernacularTitle:广东地区副溶血性弧菌暴发分离优势血清型菌株的分子特征
- Author:
Cong MA
;
Dongmei HE
;
Xiooling DENG
;
Bixia KE
;
Bosheng LI
;
Wei LI
;
Hailing TAN
;
Changwen KE
- Publication Type:Journal Article
- Keywords:
Vibrio parahaemolyticus;
Major serotypes;
Pandemic group;
Similarity
- From:
Chinese Journal of Microbiology and Immunology
2011;31(12):1093-1098
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the toxin genes and pandemic group distribution as well as the genetic correlation between the major serotypes( O3:K6,O1:Kut,O4:K8 ) of Vibrio parahaemolyticus (VP) isolated from the outbreaks of Guangdong province.MethodsThe tdh and trh genes,GS-PCR and orf8 gene were detected on the 62 isolates sourced from patient and seafood occurred in the 23 outbreaks during 2008-2010.44 isolates of which were analyzed on PFGE digested by Not Ⅰ enzyme.ResultsToxin genes distributions suggested that 96.8% (60/62)isolates were tdh+,trh-.Three tdh+ isolates sourced from seafood were found.Pandemic group distribution suggested that 97.2% (35/36) O3:K6,5.88% (1/17) O4:K8,66.7% (8/9)O1:Kut serotype was GS-PCR+ and/or orf8+,respectively.PFGE analysis suggested that 44 isolates were separated into 3 clusters,of which the similarity of PFGE profile was 80.5% in the pandemic group cluster constructed by 28 isolates,the similarity between pandemic group and non-pandemic group was 59.5%.Pandemic group of O3:K6,O1:Kut as well as 04:K8 isolated on some outbreaks were processing the same PFGE profiles.ConclusionThe characteristic of toxin genes of major serotypes VP isolated in the outbreaks of Guangdong province form 2008-2010 was tdh- present and trh- absent.Within the pandemic group,O3:K6 and O1:Kut were the major serotypes.In single outbreak,isolates belongs to pandemic group but with different serotype seem to be close correlations.
