Association of cytokine gene polymorphisms with long-term humoral response in children vaccinated against hepatitis B in infancy
10.3760/cma.j.issn.0254-5101.2011.12.016
- VernacularTitle:细胞因子基因多态性与新生儿接种乙型肝炎疫苗后远期体液免疫应答水平的关系
- Author:
Dongmei ZHU
;
Yangyang WANG
;
Shu ZHANG
;
Yali HU
;
Yihua ZHOU
- Publication Type:Journal Article
- Keywords:
Cytokine;
Gene polymorphisms;
Hepatitis B vaccine;
Anti-HBs
- From:
Chinese Journal of Microbiology and Immunology
2011;31(12):1124-1128
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the relationship between cytokine gene polymorphisms and long-term humoral response in children vaccinated against hepatitis B during infancy.MethodsA total of 293 children (6.08±0.59 years old) who received three doses of hepatitis B vaccine according to a 0-,1-,6-month schedule during infancy and were negative for HBsAg and/or anti-HBc,were enrolled.Of them,83children with anti-HBs ＜10 mIU/ml were considered as long-term poor responders ( group A),and 210 others with anti-HBs ≥ 10 mIU/ml were defined as long-term responders ( group B).A total of 11 single nucleotide polymorphisms (SNPs) of IL-1β,IL-2,IL-4,IL-10,IL-12B,and IL-13 were detected with PCR-restriction fragment length polymorphism.ResultsThe allele frequencies of -33T,-589T,and 2979T in IL-4 gene in group A were 86.1%,86.1% and 90.4%,respectively,higher than those in group B (76.0%,76.9%,and 83.3%,respectively,all P＜0.05).In IL-4 gene,frequencies of TT genotype at position -33 and -589 in group A were 74.7% and 75.9% respectively,higher than those in group B (57.1% and 59.0% respectively,both P＜0.01 ),while the frequencies of CT at position -33 and -589 in group A was 22.9% and 20.5% respectively,lower than those in group B (37.6% and 35.7% respectively,both P＜0.05).The genotype distributions of IL-4 2979 and the allele or genotype distributions of 8 other SNPs showed no significant differences between group A and group B.ConclusionPolymorphisms at position -33,-589,and 2979 in IL-4 gene are associated with the long-term humoral response in children vaccinated against hepatitis B during infancy.
