Role of HSP47 in renal tubulointerstitial fibrosis induced by transforming growth factor β1
10.3760/cma.j.issn.1001-7097.2011.12.012
- VernacularTitle:热休克蛋白47在转化生长因子β1诱导肾小管间质纤维化中的作用
- Author:
Hongbo XIAO
;
Jinlei LV
;
Qinkai CHEN
;
Ruihong LIU
;
Guanghui LING
- Publication Type:Journal Article
- Keywords:
Hot shock protein 47;
Transforming growth factor β1;
Extracellular matrix;
Renal tubular epithelial cells
- From:
Chinese Journal of Nephrology
2011;27(12):923-927
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTostudytheroleof hotshockprotein (HSP)47in tubulointerstitial fibrosis induced by transforming growth factor β1(TGF-β1),and to explore its possible mechanism.Methods Human proximal tubular epithelial cells(HK-2) were divided into threegroups:control,TGF-β1andHSP47siRNA. Theexpressionsof HSP47, collagenⅣ,fibronectin(FN),plasminogen activator inhibitor 1(PAI-1) mRNA and HSP47,collagen Ⅳ,FN protein were detected by RT-PCR and Western blotting respectively.PAl-1 protein was detected by ELISA. ResultsHK-2expressedHSP47innormalmedium. ThemRNAandprotein expressions of HSP47 up-regulated in concentration- and time-dependent manner in HK-2 cells induced with increasing concentrations of TGF-β1(0,2.5,5,10 μg/L) and with prolong times (12,24,48 h),and peaked at 10 μg/L TGF-β1 for 48 h.Similar phenomena was observed in the mRNA andproteinexpressionsof collagenⅣ, FN, PAI-1inHK-2 cellsinducedbyincreasing concentrations of TGF-β1 (0,2.5,5,10 μg/L) at different time points (12,24,48 h),and peaked at 10 μg/L TGF-β1 for 48 h.HSP47 siRNA could significantly reduce the up-regulation of mRNA and protein expressions of HSP47,collagen Ⅳ,FN,PAI-1 in HK-2 cells induced by TGF-β1.Conclusion HSP47 can promote renal tubulointerstitial fibrosis maybe through the regulation of the expressions of collagen Ⅳ,FN,PAI-1.
