Expression and biological significance of cytosolic phospholipase A2 in lung cancer tissues
10.3760/cma.j.issn.1006-9801.2011.12.007
- VernacularTitle:胞质磷脂酶A2在肺癌组织中的表达及其生物学意义
- Author:
Jingyuan WANG
- Publication Type:Journal Article
- Keywords:
Phospholipase A2;
cytosolic;
Lung neoplasms
- From:
Cancer Research and Clinic
2011;23(12):814-816
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and biological significance of cytosolic phospholipase A2(cPLA2)in lung cancer and to provide a theoretical basis for understanding the molecular mechanism of the lung cancer carcinogenesis and development.MethodsTheexpression of cPLA2 at mRNA level in tumor and tumor-adjacent tissue was detected byRT-PCR.The immunohistochemical SP method was used to evaluate the expression of cPLA2 protein in the corresponding tissues.ResultsThe quantitive expression levels of cPLA2 mRNA in tumor and tumor-adjacent tissue were0.82±0.10 and 0.08± 0.01,respectively.The mRNA level of cPLA2 significantly increased (P =0.001) in lung tumor when compared with those in the tumor-adjacent tissue. By immunohistochemistry, the positive rate of cPLA2 protein expression in lung tumor tissue was 76.7 ％(46/60),while no expression was detected in tumor-adjacent tissue.The positive rate of cPLA2 protein expression in lung cancer was correlated with clinicopathological parameters including histological type,histological grade,lymph node metastasis and TNM staging(x2 was 7.565,9.161,4.424,5.416,respectively,and all P ＜0.05),but not correlated with the size of tumor (x2 =0.366,P ＞ 0.05).ConclusionThe expression of cPLA2 in lung cancer may play an important role in the process of carcinogenesis,and may provide a clinical basis for the early diagnosis and targeted therapy for lung cancer.
