Alteration mechanics of T follicular helper cell in Kawasaki disease
10.3760/cma.j.issn.0254-5101.2011.11.016
- VernacularTitle:急性期川崎病患儿滤泡辅助性T细胞改变观察
- Author:
Fanzhen KONG
;
Chengrong LI
;
Oiu LI
;
Guobing WANG
;
Jun YANG
- Publication Type:Journal Article
- Keywords:
T follicular helper cell;
Bcl-6/Blimp-1;
IL-21;
Kawasaki disease
- From:
Chinese Journal of Microbiology and Immunology
2011;31(11):1027-1030
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the alteration mechanism of T follicular helper (Tfh) cells in patients with Kawasaki disease (KD).MethodsTwenty children with KD and the same number of agematched healthy subjects were studied.The proportion of CD4+CXCRS+ICOS+ T in peripheral blood was analyzed by flow cytometry.Real-time PCR was performed to detect the level of Tfh transcriptional factor( Bcl6) and its inhibitor( Blimp-1 ).The plasma concentration of IL-4 and IL-21 were determined by ELISA.ResultsThe proportion of CD4+CXCR5+ICOS+ T in patients with KD was significantly higher than healthy controls [ (2.6±0.6) ％ vs ( 1.8±0.7 ) ％,P＜0.05 ].Transcription levels of Bcl-6 were significantly elevated in patients with KD( P＜0.05),its inhibitor Blimp-1 were found to be down-regulated during acute phase of KD compared with healthy controls (P ＜ 0.05 ).The significant increase of IL-4 and IL-21 plasma concentrations were detected in patients with KD(P＜0.05),in comparison with healthy controls.ConclusionThe over activity of Tfh might be correlated with immune dysregulation in Kawasaki disease.Dysregulation of Bcl-6/Blimp-1,altered microenvironment of IL-4 and IL-21 might be correlated with the abnormal activity of Tfh cells.
