The transcription activity of NF-κB signal pathway down regulated by Ras homolog gene family member Ⅰ in gastric cancer cell
10.3760/cma.j.issn.0254-1432.2012.01.009
- VernacularTitle:Ras同源基因家族成员Ⅰ基因在胃癌细胞中下调核因子-κB信号转导通路转录活性
- Publication Type:Journal Article
- Keywords:
Genes,tumor suppresson;
NF-kappa B;
Stomach neoplasms;
Tumor cells,cultured;
Signal transduction;
Phosphorylation
- From:
Chinese Journal of Digestion
2012;32(1):37-41
- CountryChina
- Language:Chinese
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Abstract:
ObjectiveTo investigate the relation between aplysia Ras homolog Ⅰ(ARH Ⅰ )gene and NF-κB,and explore the possible mechanism in gastric cancer pathogenesis and development.Methods Human embryonic kidney cell line 293T cells were co-transfected with pre-constructed pIRES2-EGFP-ARH Ⅰplasmid and NF-κB luciferase reporterplasmid. The activity of NF-κB transcription was determined according to luciferase reaction.The expression of ARH Ⅰ in MKN-28 cells was interfered by siRNA. The phosphorylation level of NF-κB and the changes of NF-κB expression in nuclear was detected by Western blot.ResultsThe activity of NF-κB transcription was significantly down-regulated by ARHⅠoverexpressing in 293T cells.In MKN-28 cell line,the phosphorylation level of NF-κB and its expression in nuclear at protein level was significantly upregulated.ConclusionsThe activity of NF-κB transcription can be down-regulated by ARH Ⅰ,and which may reduce the phosphorylation level of NF-κB,and restrict it from entering the nucleus,and thus play a role in tumor suppression.
