The experimental study of inhibition of effective constituent of Giant Typhonium Rhizome on human bone marrow neuroblastoma cell (SH-SY5Y) proilferation
10.3760/cma.j.issn.1673-4246.2012.09.011
- VernacularTitle:白附子水提物抑制人骨髓神经母细胞瘤细胞增殖的实验研究
- Author:
Tongzhang YANG
;
Hongbo GAI
- Publication Type:Journal Article
- Keywords:
Giant Typhonium Rhizome;
Cell inhibition rate;
Median inhibitory concentration;
CCK-8;
Flow cytometry
- From:
International Journal of Traditional Chinese Medicine
2012;34(9):801-803
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the anti tumor effect of Giant Typhonium Rhizome.Methods SH-SY5Y was treated for 24 or 48 h by Giant Typhonium Rhizome.Cell Counting Kit-8 (CCK-8) cell proliferation and cytotoxicity assays were applied to detect the inhibition of SH-SY5Y cells treated with aqueous extract of white monkshood root.AV/PI double staining flow cytometry was applied to explore the mechanism of growth inhibition.ResultsSH-SY5Y cells could be inhibited by root aqueous extract of Giant Typhonium Rhizome,the IC50 at 48 h (50％ inhibitory concentration) was 0.862 mg/ml,while the IC50 at 24 h was 1.158 mg/ml.Western-blot results showed that root aqueous extract of Giant Typhonium Rhizome could induce apoptosis to inhibit tumor cells.ConclusionThe Rhizoma typhonii inhibit the growth of SH-SY5Y.
