AMP-activated protein kinase inhibits KiSS-1 gene expression through SP1 in the hypothalamic GT1-7 neurons

VernacularTitle:腺苷酸活化的蛋白激酶通过SP1抑制下丘脑GT1-7神经元KiSS-1基因表达的机制研究
Author: Junping WEN ; Yating HU ; Chune LIU ; Wenkai BI ; Huibin HUANG ; Jixing LIANG ; Liantao LI ; Lixiang LIN ; Gang CHEN
Publication Type:Journal Article
Keywords: KiSS-1; AMP-activated protein kinase; SP1; Translocation
From: Chinese Journal of Endocrinology and Metabolism 2012;28(9):754-757
CountryChina
Language:Chinese
Abstract: The effect of AMP-activated protein kinase (AMPK) on KiSS-1 mRNA levels was detected by realtime PCR in the hypothalamic GT1-7 neurons. The promoter activity of KiSS-1 gene was detected by DualLuciferase Reporter Assay System.The effects of AMPK on the protein expression and subcellular distribution of SP1 were determined by Western blot.The results showed that AMPK reduced the mRNA expression and promoter activity of KiSS-1 gene while SP1 increased the promoter activity of KiSS-1 gene. Besides,AMPK alse decreased the translocation of SP1.These results suggest that AMPK may inhibit the expression of KiSS-1 gene by decreasing the translocation of SP1 from cytoplasm to nucleus in the hypothalamus GT1-7 neurons.