Expression and clinical significance of dicer in hilar cholangiocarcinoma tissues and cells
10.3760/cma.j.issn.1007-8118.2012.10.008
- VernacularTitle:核酸内切酶在肝门部胆管癌组织和细胞株中的表达和临床意义
- Author:
Guangyao YANG
;
Kang YANG
;
Songqi WEN
;
Yahong YU
;
Zhiqiang DING
;
Yongjun CHEN
- Publication Type:Journal Article
- Keywords:
Dicer;
Hilar cholangiocarcinoma;
Clinicopathological characteristic;
Prognosis
- From:
Chinese Journal of Hepatobiliary Surgery
2012;18(10):753-757
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the relationship between Dicer expression and clinicopathological characteristics and prognosis by detecting the expression of Dicer in hilar cholangiocarcinoma tissues and cells.MethodsThe expression of Dicer in tissues was detected using immunohistochemistry.Western blotting and RT-PCR were used to investigate Dicer expression in QBC939 and HIBEpic cells.The relationship between Dicer expression and clinicopathological characteristics was analyzed.A Kaplan-Maier analysis was performed to analyze the disease-free survival (DFS) and overall survival (OS) after radical surgical resection of hilar cholangiocarcinoma.ResultsWhen compared to control,Dicer was significantly down-regulated in hilar cholangiocarcinoma tissues (P＜0.05) and in QBC939 (P＜0.05).The expression of Dicer was higher in well differentiated adenocarcinoma than poorly and moderately differentiated tumours. Univariate analysis showed low expression of Dicer protein was significantly correlated with short disease-free survival and overall survival of patients with hilar cholangiocarcinoma after radical surgical resection (P＜0.01). Multivariate analysis revealed that the expression of Dicer was the most important factor for predicting prognosis after radical surgical resection of hilar cholangiocarcinoma (P＜0.05).ConclusionsDicer could be used as a prognostic marker for hilar cholangiocarcinoma.
