Sorting of side population cells from breast cancer MCF-7 cell line and its biological characteristics

VernacularTitle:乳腺癌MCF-7细胞系中侧群细胞分选及其生物学特性
Author: Xin SUN ; Ping LI ; Mei ZHANG ; Jiao CHEN
Publication Type:Journal Article
Keywords: MCF-7 breast cancer stem cell; Side population cell; Proliferation
From: Chinese Journal of Primary Medicine and Pharmacy 2012;19(13):1927-1928
CountryChina
Language:Chinese
Abstract: Objective To separate the side population cells(SP) from breast cancer MCF-7 cell line,and observe its biological characteristics.Methods Flow cytometry and Hcechst 33342 dye efflux assay were used to isolate SP cells and non-SP cells from the MCF-7 cell line of human breast cancer.Tumorigenicity of the two subpopulations was observed by a soft agar cloning method.Results The results of FACS analysis indicated that (6.5 ± 0.4 ) ％of the MCF-7 cells were SP cells;The vitro colony formation rate of SP cells was(38.5 ±9.4)％,and higher than that of non-SP cells ( 8.4 ± 2.6 ) ％ ( t =5.34,P ＜ 0,05 ).Concluslon The SP cells sorted from MCF-7 cell line enriched tunor stem cells,which exhibited high tumorigenicity.It indicated that SP cells should play a principal role in breast cancer.