Effects of Yishen granule on expression of type Ⅳ collagen、 LN and FN in diabetic rats with nephropathy
10.3760/cma.j.issn.1673-4246.2012.08.007
- VernacularTitle:益肾颗粒对糖尿病肾病大鼠肾组织Ⅳ型胶原、层黏连蛋白及纤维黏连蛋白表达的影响
- Author:
Xiaohua HONG
;
Bin YANG
;
Weilin YU
;
Xiaoxia DONG
;
Hongkun LI
;
Jianxun LIU
- Publication Type:Journal Article
- Keywords:
Yishen granule;
Diabetic nephropathy;
Type Ⅳ collagen;
LN;
FN
- From:
International Journal of Traditional Chinese Medicine
2012;34(8):692-696
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of Yishen granule on the expression of type Ⅳ collagen,laminin (LN)and fibroneetin (FN)in diabetic nephropathy (DN) rats.Methods DN model rats were established by unilateral nephrectomy and intraperltoneal injection of STZ.The experimental rats were divided into six groups:normal group,DN model group,Yishen granule groups in low and high dose (8.1 g/kg、16.2 g/kg) and positive control medicine group with lotensin (0.0015 g/kg).After 16 weeks of treatment,kidney tissues were sampled and pathologically observed through Masson trichrome staining.The proteins of type Ⅳ collagen、LN and FN were determined through immunohistochemical methods.Results The type Ⅳ collagen in normal group,DN model group,Yishen granule groups in low and high dose,and positive control medicine group was (1521.22 ± 415.26),(1579.22 ± 343.26),(3402.00 ± 863.39),(2984.30 ± 674.53),(2959.15 ± 561.22),(2918.04±363.96); LN was (1968.04±522.17),(2004.52±417.19),(3299.04±665.78),(3116.89±540.10),(2932.63 ± 528.38),(2815.89 ± 798.58) ; FN was (2614.67 ± 533.82),(2742.63 ± 562.80),(3311.41 ± 529.29),(2993.44±548.66),(2953.30±535.74),(2897.41 ±505.84) respectively.The level of type Ⅳ collagen、LN and FN expression in Yishen granule group obviously decreased as compared with that in DN model group (P＜0.05).The mesangial cells,basement membrane thickening and endocapillary proliferation in glomerular filtration membrane were significantly alleviated in Yishen granule group.The pathology of kidney was obviously modulated by Yishen granule.Conclusion Yishen granule can significantly inhibits the expression of type Ⅳ collagen、LN and FN in DN rats,which may be the mechanisms for Yishen granule in protecting the DN rat's kidney.
