Effects of HIF - 1α on mitochondrial function of cardiomyocytes stimulated by lipopolysaccharide
10.3760/cma.j.issn.1671-0282.2012.08.009
- VernacularTitle:缺氧诱导因子-1α对脂多糖所致心肌细胞线粒体功能损伤的影响
- Author:
Le YANG
;
Xiaojing ZOU
- Publication Type:Journal Article
- Keywords:
Lipopolysaccharide;
Hypoxia inducible factor-1α;
Cardiomyocyte;
Mitochondrial
- From:
Chinese Journal of Emergency Medicine
2012;21(8):836-839
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effects of HIF-1α,on mitochondrial function of cardiomyocytes stimulated by lipopolysaccharide (LPS).MethodsCultured neonatal cardiomyocytes were randomly (random number) distributed to four groups (n =5).Control group:the neonatal cardiomyocytes were cultured in normal condition without any treatmeat; LPS group:cardiomyocytes were exposed to 1 μg/ml LPS for 2 h; YC-1 + LPS group:cardiomyocytes were exposed to 4 μmol/L YC-1 for 8 h before LPS treatment; YC-1 group:cardiomyocytes were exposed to 4μmol/L YC-1 for 8 h.As indexes of mitochondrial function,MMP,the content of ATP and the activity of cytochrome oxidase were measured,COX1/2 protein expression was determined by Western blotting.Results Compared with control group,LPS decreased MMP,the content of ATP and the activity of COX obviously ( P ＜0.05 ),depressed the expression of COX- 1 protein ( P ＜ 0.05 ),induced the expression of COX-2 protein ( P ＜ 0.05 ).Compared with the LPS group,MMP,the content of ATP and the activity of COX were markedly attenuated ( P ＜ 0.01 ),the expression of COX-1 was significantly increased and the expression of COX-2 was decreased in theYC-1 +LPS group (P ＜ 0.01 ).ConclusionsYC-1 attributes to the dysfunction of mitochondrial of myocyte by alterating the expression of COX-1 and 2, HIF-1α may attenuate the mitochondrial dysfunction of cadiomyocytes stimulated by LPS.
