Preliminary study of effects on dogs' testis by microbubbles excitated by ultrasound
10.3760/cma.j.issn.1004-4477.2012.08.029
- VernacularTitle:超声击破微泡对犬睾丸组织及细胞影响的研究
- Author:
Shuyi XIAO
;
Jianhua LIU
;
Wencai OU
;
Jingguang HE
;
Chunhua QIU
;
Suihong MA
;
Hong DU
- Publication Type:Journal Article
- Keywords:
Ultrasonic therapy;
Microhubbles;
Testis;
Blood-testis barrier
- From:
Chinese Journal of Ultrasonography
2012;21(8):723-726
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects on dogs' testis by microbubbles excitated by ultrasound.Methods Sixteen male dogs were divided into 3 groups randomly.Pulsed ultrasound irradiation and intravenous microbubbles injection were both applied in the microbubble enhanced ultrasound group (MEUS),pulsed ultrasound irradiation and intravenous microbubbles injection were individually applied in the ultrasound group (TUS) and the simple microbubbles group (MB).Results In MEUS group,the layers of seminiferous tubules decreased and the germ cells were arranged disorderly and bubbles appeared in the cells.The appearances of focal ultra-structural damage such as intercellular space widening,baseline broken and tight-connection of sustentacular cells disappearing were observed by transmission electron microscope,which were slightly or hardly demonstrated in other groups.Conclusions Microbubbles excitated by ultrasound can affect testicular germ cells and the integrity of bloold-testis harrier,which providing new train of thought of target treatments of testis diseases.
