The relationship between the pathological changes and neuronal function score of graded fluid percussion brain injury in rats
10.3760/cma.j.issn.1674-6554.2012.08.005
- VernacularTitle:不同冲击压力脑损伤大鼠神经功能评分差异性与病理学特征的关系
- Author:
Guozhu SUN
;
Lianqi YANG
;
Panhu CHEN
;
Zongmao ZHAO
;
Yangjun HAN
- Publication Type:Journal Article
- Keywords:
Brain injury;
Fluid percussion;
Nervous function score;
Histology
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2012;21(8):687-689
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the relationship between neuronal function score and pathological changes of fluid percussion brain injury in rats and to explore their clinical significances.MethodsThe fluid percussion models of brain injury in rats were established by using the improved device with three kinds impact pressure such as 0.1 MPa,0.2 MPa,0.3 MPa,and vital signs and mortality rate were observed.Behavior changes,brain water content,histological changes were observed by Shapira and Wahld method,dry-wet measure,light microscopy at 1 h,6 h,12 h,24 h,3 d and 7 d after operation respectively.ResultsThe animals accepted impact pressure of 0.1 MPa showed temporary hypopnea with mortality rate of 2.08％,those of 0.2 MPa suffered apnoea of ( 10.88 ±2.69 ) s with mortality rate of 4.17％ and those of 0.3 MPa suffered apnoea of ( 20.60 ± 3.02 ) s with mortality rate of 16.67％.As the impact pressure increased,nervous function score minimumly decreased to (7.17 ±0.75) of 0.1 MPa group,(4.83 ± 0.75 ) of 0.2 MPa group and (2.67 ± 0.52) of 0.3 MPa group respectively,and recovered more slowly.Brain water content maximumly reached to (81.12 ± 0.03 )％,(82.74 ± 1.11 )％ and (83.89± 0.04) ％ at time point of 24 h respectively.The brain injury was involved in the outer layer of cerebral cortex,hippocampal formation and brain stem respectively and histological observation verified above findings.Conclusion Light,moderate and heavy fluid percussion brain injury in rats have more and more low nervous function scores,which have positive relationship with more and more serious pathological changes.
