The role of CXCR3 and its ligand I-TAC in the pathogenesis of immune thrombocytopenic purpura
10.3760/cma.j.issn.0578-1426.2012.08.015
- VernacularTitle:CXC亚族趋化因子受体3及其配体I-TAC在免疫性血小板减少性紫癜发病中的作用
- Author:
Xiang ZHANG
;
Jianming FENG
;
Wenqian LI
;
Jianping LI
;
Shaobin CHEN
;
Guoxiong HAN
- Publication Type:Journal Article
- Keywords:
Interferon type Ⅱ;
Purpura,thrombocytopenic,immune;
CXCR3;
I-TAC
- From:
Chinese Journal of Internal Medicine
2012;51(8):634-637
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the roles of the chemokine receptor CXCR3 and its ligand I-TAC in the pathogenesis of immune thrombocytopenic purpura (ITP).Methods A total of 48 ITP patients were enrolled in this study:30 with newly diagnosed or relapse ITP and 18 in remission after treatment,and 24 healthy volunteers were as controls.IFNγ and I-TAC in plasma were detected by ELISA.The mRNA expression of CXCR3 in the peripheral blood mononuclear cells (PBMNCs) was determined by quantitative RT-PCR.Results The IFNγ level in the plasma of ITP patients before the treatment was obviously increased than those in the remission group and controls[ (71.45 ± 17.62)ng/L vs (36.94 ±14.86 )ng/L and (25.28 ± 12.85 )ng/L,all P ＜ 0.05 ]and those in the remission group was higher than in the controls ( P ＜ 0.05 ).In contrast,there were no statistic differences of the levels of I-TAC among the three groups[ (455.56 ± 144.70 ) ng/L,( 488.24 ± 164.70 ) ng/L and ( 382.97 ± 167.43 ) ng/L,P ＞0.05 ].Both ITP patients before the treatment and remission groups expressed more CXCR3 mRNA [ 6.76(3.03,37.00),1.76 (0.45,14.18 ) vs 0.12 ( 0.04,0.28 ),P ＜ 0.05 ].After effective therapy,CXCR3mRNA expression decreased,while it was still higher than that in the controls.Conclusions Our data demonstrate that Th1 cytokine (IFNγ) dominance is reflected in ITP.Simultaneously,the CXCR3 + cell may play a role in cell-mediated immunity through chemotaxis in ITP.
