Effect of calcitonin gene-related peptide on angiogenesis of human umbilical vein endothelial cells
10.3760/cma.j.issn.0253-2352.2012.08.013
- VernacularTitle:降钙素基因相关肽对血管内皮细胞体外血管生成的作用及机制研究
- Author:
Yonghua TUO
;
Xiaolei GUO
;
Xinxin ZHANG
;
Zhao WANG
;
Jun WEN
;
Jian ZHOU
;
Liheng XIA
;
Yongtao ZHANG
;
Dan JIN
- Publication Type:Journal Article
- Keywords:
Calcitonin gene-related peptide;
Umbilical veins;
Endothelial cells;
Tissue engineering;
Bones
- From:
Chinese Journal of Orthopaedics
2012;32(8):781-787
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of calcitonin gene-related peptide (CGRP) on angiogenesis of human umbilical vein endothelial cells (HUVECs).Methods The HUVECs were collected from human umbilical core,and the expression of the CGRP receptor-1 was identified though immunofluorescence.After HUVECs were treated with CGRP,the angiogenesis was detected through tube formation experiment.The secretion of vascular endothelial growth factor (VEGF) was detected through ELISA method.The mRNA expression of VEGF,VEGF receptor-1 (FLT1),VEGF receptor-2 (KDR) and CGRP receptor-1 were detected through quantitative PCR (Q-PCR) at 3,7,10 days after culturing.Western blot method was used to detect the protein expression of FLT1 and KDR in HUVECs.Results Immunofluorescence result showed CGRP receptor-1 expressed in HUVECs.CGRP could significantly promote angiogenesis and increase VEGF secretion in direct manner.The Q-PCR results showed that the mRNA expression level of CGRP receptor-1 was significantly higher in CGRP groups than that in control group,especially at 10 days.Compared to the control group,the mRNA and protein expression level of FLT1 and KDR were statistically higher in CGRP groups at different time.Conclusion CGRP can significantly promote angiogenesis of HUVECs in vitro,which may be because it can promote VEGF secretion and expression of FLT1 and KDR in HUVECs.Meanwhile,the increase of CGRP receptor-1 expression also can promote angiogenesis of HUVECs.
