Common bile duct ligation preconditioning alleviates renal ischemia/reperfusion injury in mice
10.3760/cma.j.issn.0254-1785.2012.08.004
- VernacularTitle:胆总管结扎预适应减轻小鼠肾脏缺血再灌注损伤
- Author:
Panliang WANG
;
Yang DONG
;
Ming ZHANG
;
Jianjun ZHANG
- Publication Type:Journal Article
- Keywords:
Common bile duct;
Ligation;
Reperfusion injury,kidney;
HSP27 Heat-shock proteins;
Nitric oxide synthase
- From:
Chinese Journal of Organ Transplantation
2012;33(8):459-461
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the possible mechanism of common bile duct ligation (CBDL) preconditioning alleviating subsequent renal ischemia/reperfusion injury.MethodsC57BL/6 mice was divided into 3 groups.In CBDL group,the common bile duct was transiently ligated at day 0 (d0),and recanalized after two days (d2) and two days later (d4). Unilateral renal ischemia/reperfusion injury was performed.Sham operation was done in Sham group at d0,then unilateral renal I/R was performed at d4. Control group was set as normal contrast. Total bilirubin (TB) was monitored.24 h after I/R (d5),we collected blood to detect creatinine (Cr),and the expression of heat-shock protein 27 (HSP-27) and endothelial nitric oxide synthase (eNOS) was monitored by Western blotting at d0,d2,d4,d5 four time points.ResultsAfter CBDL,TB fell to normal at d4.Cr levels in CBDL group were lower than in Sham group (P＜0.05),the protein expression levels of HSP-27 and eNOS were increased from d2 and sustained to d5 in CBDL group as compared with Sham group ( P ＜ 0.05 ).Conclusion CBDL preconditioning can obviously alleviate renal ischemia/reoerfusion injury in mice probably by up-regulating the expression of HSP-27 and eNOS.
