A quantitative analysis of factors affecting BK viral DNA loads in early renal transplant recipients
10.3760/cma.j.issn.0254-1785.2012.08.009
- VernacularTitle:肾移植后早期受者BK病毒DNA拷贝数的影响因素研究
- Author:
Xiaoyong YANG
;
Shihai LI
;
Liang REN
;
Wei WANG
;
Hang YIN
;
Hang LIU
;
Xiaopeng HU
;
Xiaodong ZHANG
- Publication Type:Journal Article
- Keywords:
Kidney transplantation;
BK virus;
Risk factors;
Viral load
- From:
Chinese Journal of Organ Transplantation
2012;33(8):477-480
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate DNA loads and risk factors of BK virus infection in renal transplant recipients.MethodsWe developed a real-time PCR assay to quantitate BK virus loads in 80 patients receiving renal transplantation in our center,and correlation between the BK virus load and clinical course was analyzed.BK virus loads were measured in urine and plasma. Epidemiological features and risk factors of BK virus infection were analyzed.ResultsThe positive rate of BKV viruria and viremia in 80 renal recipients was 37.5％ (30/80) and 8.75％ (7/80),respectively.BKV loads were higher in renal allograft recipients whose age was more than 50 years old.BKV loads were observed in urine and plasma (compared with group whose age was less than 50 years,P=0.017 and 0.05,respectively).BKV DNA copies were higher in group Tac than that in group CSA (P＜0.05),and the peak of BKV load in serum appeared at14th and10th month after transplantation,respectively,but the peak in urine was ahead of that in serum,appeared at 2nd and 8th month,respectively.ConclusionSerial measurement of BKV viral loads by quantitative PCR is a useful tool in monitoring the course of BK virus infection.The ages of recipients (＞50 years) and using Tac + MPA can reactivate BK virus and then result in BKVAN in renal transplant recipients. Intensive BKV monitoring is necessary for these recipients.
