Macrophage migration inhibitory factor promoter polymorphisms and predisposition to adult onset Still's disease in Chinese population

VernacularTitle:巨噬细胞移动抑制因子启动子区基因多态性与成人斯蒂尔病的关系研究
Author: Fangfang WANG ; Suli WANG ; Liangjing LV
Publication Type:Journal Article
Keywords: Still's disease,adult onset; Macrophage migration inhibitory factor; Gene-polymorphisms
From: Chinese Journal of Rheumatology 2012;16(8):508-511
CountryChina
Language:Chinese
Abstract: Objective To investigate the role of macrophage migration inhibitory factor (MIF) gene polymorphisms in adult onset Still's disease(AOSD).Methods Plasma MIF levels were measured by enzymelinked immunosorbent assay (ELISA).Genomic DNAs were collected from patients and controls.The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay and short tandem repeat genotyping were used to detect the polymorphisms of MIF gene promoter.The -173 G/C SNP and -794 CATT repeat genotype and allele frequencies between patients and controls were capared using pearson's Chi-square test.The non-parmetric Mann-Whithey U test was conducted to compare MIF expressin between cases and controls.Results Plasma MIF levels were significantly higher in AOSD (119±113) ng/ml than those in controls (55±29) ng/ml (P＜0.01).Individuals with -173 * C allele (OR=1.776; 95％CI 1.101-2.864; P=0.017) or -794 *5-CATT allele (OR=1.81;95％CI 1.27-2.58; P=0.001) were at increased risk for AOSD.Conclusion The MIF gene polymorphisms are associated with AOSD.