Recombinant human erythropoietin inhibiting apoptosis of human mesenchymal stem cells induced by H2O2 in vitro

VernacularTitle:重组人促红细胞生成素在体外抑制H2O2诱导的骨髓间充质干细胞凋亡
Author: Haihong LIN ; Hui GONG
Publication Type:Journal Article
Keywords: erythropoietin; mesenchymal stem cell; cell apoptosis; signal pathways
From: Chinese Journal of Organ Transplantation 2012;33(8):470-473
CountryChina
Language:Chinese
Abstract: ObjectiveTo investigate the effects of recombinant human erythropoietin (rhEPO) on the apoptosis of human mesenchymal stem cells induced by H2O2 and related cell signaling pathways.Methods After being cultured in vitro, human MSCs were treated with rhEPO.Phosphorylation of ERK1/2,p38 MAPK and PI3K/Akt was detected by using Western blotting.One h after pretreatment with1U/ml rhEPO,cells were cultured in the presence of1mmol/L H2O2 for1h.Cell morphology was observed under the inverted microscopy.Cell apoptosis was determined by using flow cytometry,migration assay was performed in Transwell chambers,and adhesion assay was performed by plastic dishes.ResultsRhEPO could increase phosphorylation of PI3K/Akt pathway in human MSCs,but reduce phosphorylation of p38MAPK.RhEPO had no obvious effect on ERK1/2 pathway and total proteins of Akt and p38MAPK.RhEPO could decrease apoptosis of human MSCs induced by H2O2 (P＜0.01) and the inhibitory effect was abrogated by Ly294002 but not anisomysin.Conclusion RhEPO could protect MSCs from apoptosis. Activation of PI3K/Akt pathway was involved in the effect of rhEPO on apoptosis.