Detection and distribution of Helicobacter pylori antibodies by protein chip in gastroduodenal disease

Meihua Cui; Xiuli Zhang; Xinyan Zhang; Guoxing YI; Youpeng Yang; Yi FU; Lin Yue; Fanghong Mou; Guibin Yang

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Detection and distribution of Helicobacter pylori antibodies by protein chip in gastroduodenal disease

VernacularTitle:蛋白芯片技术检测幽门螺杆菌抗体在胃十二指肠疾病中的分布研究
Author: Meihua CUI ; Xiuli ZHANG ; Xinyan ZHANG ; Guoxing YI ; Youpeng YANG ; Yi FU ; Lin YUE ; Fanghong MOU ; Guibin YANG
Publication Type:Journal Article
Keywords: Stomach diseases/metabolism/blood; Duodenal diseases/metabolism/blood; Helicobacter pylori/virology; Protein array analysis
From: Journal of Chinese Physician 2012;14(7):865-867
CountryChina
Language:Chinese
Abstract: Objective To detect Helicobacter pylori (H.pylori) multiple antibodies of urease (Ure),cytotoxin associated gene A protein (CagA),vacuolating toxin A (VacA),heat shock protein 60 (Hap60) and nitroreductase ( RdxA),and disclose their relations with chronic gastritis,peptic ulcer and gastric cancer.Methods A volume (3 ml) of venous blood was taken from 300 patients of gastroduodenal disease diagnosed by endoscopy,to centrifuge and detect antibodies of Ure,CagA,VacA,Hsp60,RdxA by protein chip technique.Results The infective rates of H.pylori in chronic gastritis,peptic ulcer,and gastric cancer were 34.0％,58.0％,34.0％ ( P ＜ 0.01 ),respectively.In the H.pylori positive chronic gastritis,peptic ulcer and gastric cancer,the positive rates of CagA antibody were 54.9％,75.9％,64.7％ ( P =0.070) ; the positive rates of VacA antibody were 31.4％,22.4％,17.6％ ( P =0.412) ;and the positive rates of Hap60 antibody were 56.9％,48.3％,41.2％ ( P =0.466),respectively.The total positive rate of RdxA antibody was 4.0％ (5/126).Conclusions H.pylori infection and virulence factor CagA are closely related to peptic ulcer,while it did not show the exact correlation between VacA,Hsp60 and gastroduodenal disease.The level of RdxA antibody can not represent the level of H.pylori resistance to metronidazole.