ox-LDL enhances the mRNA expressions of MMP-9 and LOX-1 in human umbilical endothelial cells and how LOX-1 acts as in inflammation course

VernacularTitle:氧化低密度脂蛋白促进内皮细胞MMP-9 mRNA、LOX-1 mRNA的表达及LOX-1在其中的作用研究
Author: Hezhong ZHU ; Xiaoyu SHEN ; Jiajuan CHEN ; Qingmin PAN ; Jie YAN
Publication Type:Journal Article
Keywords: Lipoproteins,LDL/pharmacology; Matrix metalloproteinase 9/drug effects/genetics; Receptors,LDL/drug effects/genetics
From: Journal of Chinese Physician 2012;14(7):920-922
CountryChina
Language:Chinese
Abstract: Objective To observe how ox-LDL impacts the mRNA expressions of MMP-9 and LOX-1 of human umbilical endothelial cells (HUEVC) and how LOX-1 acts as in inflamation course.Methods HUVEC were incubated in vitro.mRNA Expressions of MMP-9 and LOX-1 were determined by reverse transcription polymerase chain reaction (RT-PCR).Results Compared to the control group(0.252±0.032;0.279 ±0.041 ),ox-LDL significantly increased the mRNA expressions of MMP-9 and LOX-1 (25 ng/group 0.486 ± 0.012,0.586 ± 0.02;50 ng/L group 0.668 ± 0.011,0.739 ± 0.014; 100 ng/group 0.817 ±0.030,0.872 ±0.003,P ＜0.01 ).Those expressions were increased by ox-LDL( 1.020 ±0.039)in a concentration-and time-dependent manner.MMP-9 mRNA(0.872 ±0.046) was reduced when LOX-1 was inhibited by polyinosinic acid ( P ＜ 0.01 ).Conclusions The mRNA expressions of MMP-9 and LOX-1 were induced by ox-LDL in HUVEC.Inhibition of LOX-1 may decrease the expression of MMP-9.Those data demonstrate that LOX-1 is involved in the process of ox-LDL-induced MMP-9 expression.