The chimerism status of bone marrow mesenchymal stem cells following double-unit cord blood stem cell transplantation
10.3760/cma.j.issn.0254-1785.2012.08.015
- VernacularTitle:双份脐血移植后受者骨髓间充质干细胞嵌合情况
- Author:
Hong TIAN
;
Guanghua CHEN
;
Depei WU
;
Yang XU
;
Man QIAO
;
Huiwen LIU
- Publication Type:Journal Article
- Keywords:
Double-unit cord blood stem cell transplantation;
Bone marrow mesenchymal stem cells;
Chimerism status
- From:
Chinese Journal of Organ Transplantation
2012;33(8):498-502
- CountryChina
- Language:Chinese
-
Abstract:
ObjcetiveTo study the chimerism status ot bone marrow mesenchymal stem cells (BMSC) following double-unit cord blood stem cell transplantation (DCBT).MethodsBone marrow mononuclear cells were isolated by ficoll density gradient centrifugation from the patient following DCBT and healthy donors.After cultured for about 20 days,BMSC surface makers,the ability to differentiate into adipocytes and osteoblast and the expression of hematopoietic and immune molecules were detected,moreover,chimerism status of BMSC were also evaluated by STR-PCR.ResultsMSC derived from the patient were similar to normal adult BMSC in morphology,surface and differentiation ability.Besides,MSC from the patient can express a variety of hematopoietic and immune molecules normally.The results of STR-PCR showed that the patient demonstrated complete donor chimerism,hematopoiesis in BM and PB were from a single unit cord blood (donor chimerism was 96.4％ and 95.7％,respectively).But the predominant cord blood chimerism of BMSC was 5.4％,the remaining part was from the patient himself.ConclusionSustained hematopoiesis was derived from a single unit cord blood following DCBT.The majority of BMSC were origin from host tissue,mixed chimerism donor part of MSC stemed from the engrafted unit of cord blood.
