The effect of metformin on glucose metabolism of rat myoblast with high fat environment
10.3760/cma.j.issn.1008-1372.2012.07.005
- VernacularTitle:二甲双胍对高脂环境下大鼠成肌细胞糖代谢的影响
- Author:
Lingling HAN
;
Xiaolei ZHANG
;
Jia LI
;
Guoliang LIU
- Publication Type:Journal Article
- Keywords:
Metformin/pharmacology;
Palmitic acid/pharmacology;
Myoblasts/metabolism;
Glucose/metabolism;
Rats
- From:
Journal of Chinese Physician
2012;14(7):878-880
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of mefformin on glucose metabolism of rat myoblast with high fat environment.Methods The skeletal muscles of suckling Wister rats were subjected to primary culture.The myoblasts of passage 5 were divided into 5 groups:The control group was cultured in DMEM with 0.5％ BSA for 12 h.The high-fat group was exposed to 0.3 mmol/L palmitic acid for 12 h.On the basis of high-fat treatment,three intervention groups were added with 2.5,5.0 and 7.5 μg/ml metformin,respectively,for additional 24 h.Isotope tracer method was conducted to show the influence on 2-dexoy-D-[3H]glucose uptake of rat myoblast.Results With the increasing concentration of mefformin,[3H]-G uptake elevated [ ( 1.83 ± 0.34) 10-2pmd/( min · g) vs (2.33 ± 0.47 ) 10-2 pmd/( min · g),P ＜ 0.05 ],and the group of 7.5 μg/ml metformin uptakes the most.Conclusions Metformin can upregulate the glucose-uptake in rat myoblasts under the environment of high fat,and it shows a dose-dependent relationship.
