Effects of high-fat diet and rosiglitazone on AGF mRNA expression in liver of C57BL/6 mice

VernacularTitle:高脂饮食及罗格列酮对C57BL/6小鼠肝脏AGF mRNA表达的影响
Author: Qi HUANG ; Yancheng XU
Publication Type:Journal Article
Keywords: Dietary fats/adverse effects; Thiazolidinediones/pharmacology; Liver/metabolism; Angiopietin-related growth factor/metabolism/genetics; Mice
From: Journal of Chinese Physician 2012;14(7):907-909
CountryChina
Language:Chinese
Abstract: Objective To study the correlation between diabetes and the new discovered angiopietin-related growth factor (AGF)that participates in energy metabolism.MethodsA total of 24 male C.57BL/6 mice were divided into three groups:controlled group; fed with high-fat diet( HFD); fed with high-fat diet for 10 weeks and then treated with rosiglitazone ( HFD + RSG) ; The relative level of AGF mRNA expression in livers was measured by determining a ratio of PCR products of AGF to that of β-actin gene.Oral glucose tolerance test,and insulin resistance index (HOMA-IR) were used to measure blood glucose level and insulin sensitivity.Results Compared with the controls,the expression of AGF mRNA in liver decreased significantly in HFD group( P ＜0.05),and increased significantly in HFD + RSG group( P＜0.01 ).A negative correlation was found between AGF mRNA expression in mice livers and HOMA score ( r =-0.516,P ＜ 0.05 ).Conclusions AGF as a new hepatocyte derived circulating factor counteracts obesity and is related to insulin resistance.