Multiple differentiation potential of mesenchymal stem cells in nucleus pulposus of different sources:a comparative study
10.3760/cma.j.issn.0253-2352.2012.07.015
- VernacularTitle:不同来源椎间盘髓核间质干细胞特性及其多向分化能力研究
- Author:
Xiaoming GUAN
;
Xun MA
;
Li ZHANG
;
Haoyu FENG
;
Sheng ZHAO
;
Wenhui SONG
;
Liming HE
- Publication Type:Journal Article
- Keywords:
Intervertebral disk;
Mesenchymal stem cells;
Cell differentiation
- From:
Chinese Journal of Orthopaedics
2012;32(7):686-692
- CountryChina
- Language:Chinese
-
Abstract:
Objective To differential the biologic characteristics and multiple differentiation potential of mesenchymal stem cells in nucleus pulposus in (NP-MSC) scoliosis patient and patient with degenerative interverthral disc.Methods The human nucleus pulposus-mesenchymal stem cells were isolated and cultured with enzyme digestion from 2 patients of scoliosis and 2 patient with degenerative intervertbral disc separately.Cellular proliferation was detected with MTT assay and trypan blue.The immunophenotype expression of NP-MSC was detected by flow cytometry in scoliosis and degenerative group.The multiple differentiation ability of cells was assessed respectively using alizarin red dye,Oil red O dye and immunohistochemical staining.Results The primary cell morphology of scoliosis NP-MSC was the shape of spindle,while the degenerative NP-MSC was inhomogeneous.However,both of them became spindle shape after passages.The scoliosis NP-MSC was stronger than degenerative one in metabolic activity and proliferation.The percentage of positive antigen expression of CD44,CD105 and CD29 was 97％-100％ in scoliosis NP-MSC group,but 88.7％-97％ in the degenerative group.The expression of mature cell marker CD24 was negative in both groups.Furthermore,the MSC i.isolated from both groups differentiated along the osteogenic,chondrogenic but not adipogenic lineages.Conclusion The scoliosis and degenerative NP contains mesenchymal stem cells.Moreover the scoliosis NP-MSC had stronger ability of cell metabolic activity and proliferation.These cells have multiple differentiation potential with the exception of their adipogenic differentiation ability.
