Impacts of platelet-derived growth factor-D on prostate cancer cells migration
10.3760/ema.j.issn.1000-6702.2012.07.020
- VernacularTitle:血小板源性生长因子-D影响前列腺癌细胞迁移及相关机制的研究
- Author:
Qiang BU
;
Minghui ZENG
;
Dongwen WANG
;
Hua JIANG
;
Xiaoming YU
;
Aibin WU
;
Yun WU
;
Dongfang JIANG
- Publication Type:Journal Article
- Keywords:
PDGF-D;
Prostate cancer cells;
VEGF;
MMP-9
- From:
Chinese Journal of Urology
2012;33(7):544-548
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of platelet-derived growth factor-D (PDGF-D) on the prostate cancer cells migration and its possible mechanism. Methods The expressions of PDGF-D in LNCaP and PC-3 cells were detected with western blot.PDGF-D siRNA was synthesized according to mRNA sequence of PDGF-D gene and was transfected into PC-3 cell.The cells were treated with PDGF-D and PDGF-D siRNA,the cell migration was examined by Boyden chamber migration assay.The expression changes of VEGF and MMP-9 mRNA were detected by RT-PCR. Results The results of western blot indicated that the PDGF-D protein expression level was lower in LNCaP cells (29.47 ± 1.68) than that in PC-3 cells (63.43 ±2.10),(P ＜ 0.05).PDGF-D siRNA could down-regulate the PDGF-D protein expression in the transfected group (35.19 ± 1.51).The exogenous PDGF-D could promote migration of LNCaP and PC-3cells,and up-regulate the expression of VEGF,MMP-9 mRNA in PC-3 cells (P ＜ 0.05,compared with control group).PDGF-D siRNA inhibited PC-3 cells' migration and decreased the level of VEGF,MMP-9mRNA expression (0.72 ± 0.09 vs 0.43 ± 0.18,0.65 ±0.07 vs 0.22 ± 0.08) (P ＜ 0.05). Conclusion PDGF-D is involved in the promotion of prostate cancer invasion and angiogenesis.
