Inhibition effect on prostate cancer cells by an hTERT-promoter-dependent oncolytic adenovirus that expresses apoptin
10.3760/cma.j.issn.1000-6702.2012.07.022
- VernacularTitle:双特异性抗肿瘤重组腺病毒对前列腺癌细胞的抑制作用
- Author:
Jinhui WANG
;
Muchun ZHANG
;
Xiao LI
;
Yanxin QI
;
Guangchen LIU
;
Dandan SUN
;
Ningyi JIN
- Publication Type:Journal Article
- Keywords:
Prostatic cancer;
Apoptin;
Recombinant adenovirus;
Apoptosis
- From:
Chinese Journal of Urology
2012;33(7):549-553
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibition effects of an hTERT-promoter-dependent oncolytic adenovirus Ad-VT that expresses apoptin on human prostatic carcinoma cell PC-3. Methods MTT assay was used to measure viability of PC-3 cell which was infected by recombinant adenovirus.The viability was measured at time points of 12,24,36,48,60,72,84 and 96 h after infection.AO/EB staining,DAPI staining,Annexin V assay were used to investigate the lethal effect and style of Ad-VT on PC-3 cell in vitro.The Caspases were measured by whole cell extraction of PC-3 cells 48hrs after infection. Results Ad-VT,Ad-VP3 and Ad-GT inhibited the proliferation of PC-3 cell in vitro.Ad-VT and Ad-GT were more effective than Ad-VP3 on cell growth,P ＜ 0.05.At 48,72,96 h time points,the inhibition effect of Ad-VT on PC-3 cell exhibited a dose related manner.When infection at MOI 100,the inhibition effect of Ad-VT on PC-3 cells exhibited time related manner.The AO/EB staining,DAPI staining,Annexin V assay,Annexin V assays and Caspase assays showed that Ad-VT inhibited the proliferation of PC-3 cells by inducing apoptosis of prostate cancer cells,Loss of cytoplasmic membrane integrity. Conclusions The hTERT-promoterdependent oncolytic adenovirus Ad-VT could effectively suppress prostate cancer cells PC-3 growth.
