Role of δ receptor in reduction of hypoxia-reoxygenation injury to cardiomyocytes by morphine preconditioning in rats with chronic heart failure
10.3760/cma.j.issn.0254-1416.2012.07.030
- VernacularTitle:δ受体在吗啡预处理减轻慢性心力衰竭大鼠心肌细胞缺氧复氧损伤中的作用
- Author:
Chunxia HUANG
;
Ye ZHANG
;
Bin WANG
;
Shan GAO
;
Zhiwu CHEN
- Publication Type:Journal Article
- Keywords:
Receptors,opioid,delta;
Morphine;
Cell hypoxia;
Ischemic preconditioning;
Heart failure;
Myocardial reperfusion injury
- From:
Chinese Journal of Anesthesiology
2012;32(7):880-882
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the role of δ receptor in reduction of hypoxia-reoxygenation (H/R) injury to cardiomyocytes by morphine preconditioning in rats with chronic heart failure.Methods Adult male Sprauge-Dawley rats weighing 220-250 g were used in the study.Chronic heart failure was induced by injection of adriamycin 2 mg/kg via the tail vein once a week for 6 weeks.Their hearts were removed 2 weeks after the last injection and the cardiomyocytes were isolated and cultured.The cells were randomly divided into 5 groups ( n =8 each):control group (group C); H/R group; morphine preconditioning group (group MPC); morphine preconditioning + naloxone (opioid receptor antagonist ) group (group MPC + Naloxone) ; morphine preconditioning + naltrindole ( δ receptor antagonist) group ( MPC + Naltrindole group).The cells were cultured in normal culture atmosphere in group C and were exposed in hypoxic air for 3 h followed by 1 h reoxygenation in the other groups.Morphine preconditioning was performed immediately before hypoxia in group MPC.Naloxone and naltrindole were added before morphine preconditioning in groups MPC + Naloxone and MPC + Natrindole respectively.At 1 h of reoxygenation,the cell viability ( by MTT assay),activities of lactate dehydrogenase ( LDH ) and creatine kinase (CK),and cell apoptosis were detected.The apoptotic rate was calculated.Results The cell viability was significantly lower,and the activities of LDH and CK and apoptotic rate were significantly higher in groups H/R,MPC + Naloxone and MPC + Natrindole than in group C (P ＜ 0.05).The cell viability was significantly higher,and the activities of LDH and CK and apoptotic rate were significantly lower in group MPC than in group H/R ( P ＜ 0.05).Conclusion Morphine preconditioning reduces H/R injury to cardiomyocytes through activating δ receptor in rats with chronic heart failure.
