Generation and identification of pluripotent stem cells from NOD mouse
10.3760/cma.j.issn.1000-6699.2012.06.007
- VernacularTitle:NOD鼠来源诱导性多能干细胞系的建立及其鉴定
- Author:
Haiming LIU
;
Zhen ZHANG
;
Li YANG
;
Rui YANG
;
Xiaoyan YANG
;
Rongping CHEN
;
Jia SUN
;
Lifeng WU
;
Kun WU
;
Hong CHEN
;
Zhen HUANG
;
Dehong CAI
;
Hua ZHANG
- Publication Type:Journal Article
- Keywords:
Induced pluripotent stem cells;
NOD mice;
Diabetes mellitus,experimental;
Disease model
- From:
Chinese Journal of Endocrinology and Metabolism
2012;28(6):472-474
- CountryChina
- Language:Chinese
-
Abstract:
Three factors ( Oct4,Sox2,and Klf4) were introduced into tail tip-derived fibroblasts of NOD mouse by retrovirus infection.The induced pluripotent stem cells ( iPSCs ) generated from this method were analyzed in several aspects,including surface antigens,gene expression,alkaline phosphatase activity,and teratoma formation assays.The NOD mouse iPSCs generated from this study had ES-like characters,expressed ES-specific surface antigens,and possessed the ability to differentiate into 3-germ layers.Such NOD mouse-iPSCs should be useful in tyre 1 dialectic disease modeling,as well as for cell replacement therapy.
