The expression of T cell immune-related gene mRNAs in peripheral blood mononuclear cells frompatients with venous thromboembolism
10.3760/cma.j.issn.0578-1426.2012.07.014
- VernacularTitle:静脉血栓栓塞症患者外周血单个核细胞T细胞免疫功能相关基因mRNA表达
- Author:
Haoming SONG
;
Zhu GONG
;
Lemin WANG
;
Xiaoyu ZHANG
- Publication Type:Journal Article
- Keywords:
Venous thromboembolism;
T-cell Antigen receptor specificity;
RNA,messenger;
Microchip analytical procedures
- From:
Chinese Journal of Internal Medicine
2012;51(7):551-553
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of T cell-mediated immunity in the pathogenesis of venous thromboembolism ( VTE ) by analyzing the differential expression of T cell immune-related gene mRNAs peripheral blood mononuclear cells (PBMCs) between VTE patients and controls with GeneChip Human Genome. Methods Human eDNA microarray analysis was employed in PBMCs from 20 VTE patients and 20 hypertensive controls,and random variant model (RVM) corrected t-test was used for statistical analysis of differential gene expression.Results Six mRNA stripes including CD247,CD3D,CD3G,Granzyme A (GzmA),Granzyme B (GzmB) and Zeta-chain-associated protein kinase 70 (ZAP70)were found to be associated with T cell-mediated immunity.Significant down-regulation of these six mRNAs was found in the VTE group compared with the controls ( 15.3050 ± 0.6346 vs 15.8053 ± 0.5567,13.7878 ±0.7731 vs 14.3820 ±0.4857,13.3299 ± 0.9104 vs 14.1246 ± 0.6011,14.8893 ± 0.8675 vs 15.5305 ±0.4624,15.9113 ±0.8123 vs 16.4553 ±0.5055,14.3652 ±0.7717 vs 14.3652 ±0.7717;all P values ＜ 0.05 ).Conclusions T cells' function including antigen recognition,signal transduction and cytotoxicity was impaired in VTE patients.T cell-mediated immunity dysfunction probably plays an important role in the pathogenesis of VTE.
