Effect of YC-1 on improving hypoxia and radiosensitizing human lung adenocarcinoma cellsin vitro

VernacularTitle:YC-1改善乏氧增强人肺腺癌A549细胞放射敏感性的体外研究
Author: Qin CNEN ; Yun QIAO ; Peng DAI ; Jin WU ; Daan SONG ; Xiaodong JIANG
Publication Type:Journal Article
Keywords: 3-(5'-hydroxy-2'-furyl)-1-benzyl indazole; Radiosensitivity agents; A549 cell line
From: Chinese Journal of Radiation Oncology 2012;21(4):396-399
CountryChina
Language:Chinese
Abstract: ObjectiveTo investigate the radiosensitizing effect of 3-(5'-hydroxy-2'-furyl)-1-benzyl indazole ( YC-1 ) on hypoxic human adenocarcinoma cell line A549.MethodsMTT assay was used to test the inhibitory effect of YC-1 on proliferation of A549 cells.Clonogenic assay was performed to determine the radiosensitizing effect of YC-1 on hopxic A549 cells.Single-hit multi-target model was used to plot survival curve and calculate sensitization enhancement ratio (SER).The cell cycle and apoptosis were measured by flow cytometry.ResultsThe proliferation of A549 cells was inhibited by YC-1 in a time-dose-dependent manner.In normoxic and hypoxic cells,the IC20 was 16.7 μmol/L and 39.2 μmol/L at 24 h,respectively.In the group of hypoxia plus YC-1,SERD0 and SERDq were 1.11 and 1.26,respectively.In hypoxia,YC-1combined with 2 Gy irradiation could induce cell apoptosis and prolong G2 + M phase arrest ( ( 30.17 ±1.21 )％ ∶ ( 15.44 ±0.96) ％,P =0.000; (21.56 ±0.47 )％ ∶ (6.16 ±0.16)％,P =0.000).Concinsions YC-1 could enhance the radiosensitivity of hypoxic A549 cells.