Establishment of urinary proteome and functional analysis of urinary differential proteins in patients with metabolic syndrome
10.3760/cma.j.issn.1674-635X.2012.03.003
- VernacularTitle:代谢综合征患者尿蛋白质组的建立及差异蛋白功能分析
- Author:
Xuejiao LIU
;
Mingxi LI
;
Bixia GAO
;
Xiaohong FAN
;
Jianfang CAI
;
Youhe GAO
;
Xuemei LI
;
Xuewang LI
- Publication Type:Journal Article
- Keywords:
Metabolic syndrome;
Urinary proteomics;
Spectral counting;
Function analysis of proteins
- From:
Chinese Journal of Clinical Nutrition
2012;20(3):143-148
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo establish the urinary proteome profile of the metabolic syndrome ( MetS ) patients,compare the different urinary proteins between the MetS patients and the normal individuals,and analyze the function of the different proteins,so as to explore the pathogenesis of MetS.MethodsOvernight urine were collected from normal controls (n =6) and MetS patients ( n =6).Acetone precipitation method was used to precipitate proteins of urine.Intra-group proteins were mixed together,identified by reversed phase liquid chromatography-mass spectrometry/mass spectrometry and quantified relatively using spectral counting method.The functions of differential proteins were analyzed using Panther.ResultsA total of 807 and 630 proteins were identified respectively in normal controls and MetS patients.Comparing MetS patients with normal controls,sixty different proteins were found,of which 23 proteins were up-regulated and 37 proteins were down-regulated in MetS patients.In the up-regulated proteins,plasminogen was involved in the plasminogen activation cascade and isoform of alphaenolase,phosphoglycerate kinase 1 and fructose-bisphosphate aldolase B down-regulated in MetS patients were involved in the process of glycolysis and fructose metabolism.ConclusionsThe urinary proteome profile of patients with MetS was established by reversed phase liquid chromatography-mass spectrometry/mass spectrometry.Different proteins between MetS patients and normal people were identified.The plasminogen activation cascade,glycolysis and fructose metabolism play key roles in the pathogenesis of MetS.
