Gene expression profile of the differentiation of mouse embryonic stem cells into pancreatic endocrine progenitor cells in vitro
10.3760/cma.j.issn.1000-6699.2012.06.005
- VernacularTitle:胚胎干细胞体外定向分化为Ngn3+胰岛祖细胞的基因表达谱分析
- Author:
Jing SUI
;
Fangxu JIANG
;
Bingyin SHI
- Publication Type:Journal Article
- Keywords:
Embryonic stem cells;
Pancreatic differentiation;
Islet progenitor cells;
Microarray analysis
- From:
Chinese Journal of Endocrinology and Metabolism
2012;28(6):460-466
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the implications of gene expression pattern of pancreatic endocrine differentiation from mouse embryonic stem cells (mES) in vitro.Methods Mouse ES cell line ES W9.5 was cultured and differentiated into pancreatic endoerine progenitor cells by using the stepwise differentiation strategy in vitro,then stage specific genes expression were tested by RT-PCR and immnunoeytochemistry.The Illumina Mouse Ref-8 vl.1 Expression BeadChips were used to compare the changes in the transcriptome of mES-derived differentiated cells on day4,8,15,20,22,25 after differentiation to undifferentiated mES cells.Results A total 86 differentially expressed genes,both up- and down-regulated,were found during the whole differentiation stage,6 gene clusters with the same developing patterns and differentially expressed genes in 6 groups were collected.By number,the most differentially expressed genes corresponded to posterior foregut stage( 201 genes) followed by definitive endoderm stage ( 17 genes).Conclusions The analysis of the genes with the same developing pattern and differentially expressed genes at each differentiation stage during pancreas development by microarray analysis provides some evidences of early embryonic and pancreatic development.
