Comparative proteomic analysis on radioresistant nasopharyngeal carcinoma cell
10.3760/cma.j.issn.0254-5098.2012.03.006
- VernacularTitle:鼻咽癌细胞辐射抗拒的比较蛋白质组学分析
- Author:
Xiaodong ZHU
;
Shiting HUANG
;
Song QU
;
Fang SU
;
Ya GUO
;
Jinzi WANG
- Publication Type:Journal Article
- Keywords:
Nasopharyngeal carcinoma;
Radioresistance;
Comparative proteomics
- From:
Chinese Journal of Radiological Medicine and Protection
2012;32(3):245-248
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discover radioresistance-associated proteins by performing comparative proteomic analysis on nasopharyngeal carcinoma cell lines.Methods The total proteins were extracted from radioresistant human nasopharyngeal carcinoma cell line CNE-2R and its parental cell line CNE-2,respectively.These proteins were separated by high quality two-dimensional polyacrylamide gel electrophoresis (2-DE) and then the 2-DE profiles were screened for differentially expressed protein spots by the Image Master 5.0 software.Those spots were identified by a matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry.Results 32 significantly differentially expressed protein spots were screened in two different radiosensitivity cell lines and 11 proteins were identified by tandem mass spectrometry,among which 3 proteins were up-regulated in radioresistant human nasopharyngcal carcinoma cell line CNE-2R and the other 8 proteins were down-regulated.Conclusions The differentially expressed proteins of nasopharyegeal carcinoma cells with different radiosensitivity were mainly involved in apoptosis regulation,DNA damage and repair,cell cycle regulation,RNA transcription,cell signaling,cytoskeleton formation and radiation stress responses.
