Effects of accumulated 6aCo γ-ray irradiation on the small molecular metabolites in rats urine
10.3760/cma.j.issn.0254-5098.2012.04.006
- VernacularTitle:60Co γ射线累积照射对大鼠尿液中小分子代谢物的影响
- Author:
Huifang ZHANG
;
Biao YANG
;
Xuhong DANG
;
Jiangong LIU
;
Lihong XING
;
Yuefeng GUO
;
Yahui ZUO
;
Zhikai DUAN
;
Zhanqi LIU
- Publication Type:Journal Article
- Keywords:
Accumulated irradiation;
Urinary metabolites;
Nuclear resonance spectroscopy;
Principal component analysis
- From:
Chinese Journal of Radiological Medicine and Protection
2012;32(4):358-362
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of accumulated 60CD,γ-ray irradiation on small molecular metabolites in rats urine.Methods Ten healthy male SD rats were irradiated by 60Co γ-rays in 5 days and the accumulated doses were 0,0.2,0.4,0.6,0.8,1.0 Gy,respectively.The metabolites in urine of different groups were measured with 1 H-NMR combined with principal components analysis (PCA) and partial least square discriminate analysis (PLS-DA). Results The metabolites in rat urine were obviously changed after irradiation. Compared with control group,the amount of acetoacetate decreased after irradiation(t =29.7 -30.7,P ＜ 0.05 ),but its relative level was stable when the dose increased ( P ＞ 0.05 ).Meanwhile,the relative level of hippuric acid increased ( t =4.4 - 21.6,P ＜ 0.05 ) especially when the accumulated dose was higher than 1 Gy (t =21.6,P＜0.05). The relative level of proline,taurine and trimethylamine-N-oxide increased after irradiation with the same trend( t =3.5 - 13.4,4.7 - 11.5,2.9- 12.7,P＜0.05). Conclusions The acetoacetate,hippuric acid,proline,taurine,and trimethylamine-N-oxide may be applicable for biomarkers of accumulative irradiation on rat.
