Ionizing radiation activates the HIF-lα-Survivin pathway in human lymphoma cells
10.3760/cma.j.issn.0254-5098.2012.03.013
- VernacularTitle:电离辐射诱导HIF-1α-Survivin通路在人淋巴瘤细胞中的活化
- Author:
Qiao QIAO
;
Guang LI
- Publication Type:Journal Article
- Keywords:
Lymphoma;
Hypoxia inducible factor-1α;
Survivin;
Ionizing radiation
- From:
Chinese Journal of Radiological Medicine and Protection
2012;32(3):274-277
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the levels of radiation-induced expressions of HIF-1α and Survivin in three human lymphoma cell lines with or without HIF-1a specific antagonist or HIF-1a siRNA transfection and to explore the possible mechanism of cell radioresistance.Methods Namalwa,Ramos,Raji cells were irradiated with X-rays with or without the combination of echinomycin (EC),a HIF-1α specific inhibitor,or transfection of HIF-1α siRNA.Then the expression levels of HIF-1α and Survivin proteins and mRNAs were determined by Western blot and real-time PCR,respectively.Results After irradiation,the expression level of HIF-1α protein was up-regulated at 10-20 h followed by up-regulation of survivin protein at 24 h (t =7.53-31.31,P ＜ 0.01 ) in three lymphoma cell lines.Radiation-induced up-expression of survivin protein was significantly reduced by either HIF-1α inhibition or EC treatment (t =7.21-32.81,P ＜0.01 ).However,radiation failed to induce up-erpression of survivin at both mRNA and protein levels after transfection with HIF-1α siRNA.Conclusion HIF-1α-Survivin pathway is activated in human lymphoma cells after radiation,which might be involved in the cell radioresistance.
