Effect of fluoxetine on the viability and lipopolysaccharide-induced tumor necrosis factor-α release of primary cultured rat astrocytes
10.3760/cma.j.issn.1674-6554.2012.06.010
- VernacularTitle:氟西汀对体外培养星形胶质细胞活性及脂多糖诱导的肿瘤坏死因子-α释放的影响
- Author:
Aiguo DONG
;
Lili ZHAO
;
Haihong LI
- Publication Type:Journal Article
- Keywords:
Fluoxetine;
Astrocyte;
Tumor necrosis factor-α;
Depression
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2012;21(6):512-514
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effects of fluoxetine on viability and lipopolysaccharide (LPS)-induced TNF-α release of primary cultured rat astrocytes.MethodsThe cells were plated on 96-well tissue culture plates and treated with (5,10,20,40) μM fluoxetine for 24 hours,MTT method was used to measure the cell viability.The cells were plated on 48-well tissue culture plates,treated sequentially with (5,10,20,40) μM fluoxetine and 1 μg/ml LPS,and enzymatic linked immunosorbent assay(ELISA) was used to detect the TNF-α level of the cell supematant.ResultsCompared to viability of the control group( OD value:0.20 ± 0.017 ),fluoxetine at the dose of 20 μM,40 μM increased the viability of astrocytes ( OD value:0.23 ± 0.013,0.24 ± 0.012 ) (P ＜0.05,P＜0.01 ).Treatment with LPS for 24 hours,the level of TNF-α was significantly increased ( 53.84 ±24.84) pg/ml compared to the control group( 8.00 ± 10.87)pg/ml (P ＜ 0.01 ),fluoxetine at a dose of 10 μM can suppressed LPS-induced TNF-α release from astrocytes ( 28.85 ± 3.36 ) pg/ml (P ＜ 0.05 ).ConclusionFluoxetine can increase astrocytes viability and suppress LPS-induced TNF-α release from astrocytes.
