Expressions of mir-132, mir-134 in the different regions of rat brain after sleep deprivation
10.3760/cma.j.issn.1674-6554.2012.06.011
- VernacularTitle:睡眠剥夺对大鼠不同脑区microRNA-132microRNA-134表达的影响
- Author:
Han RONG
;
Tiebang LIU
;
Haichen YANG
;
Fei FENG
;
Dan XU
;
Jingjing LIU
;
Jian ZHANG
;
Qijie SHEN
- Publication Type:Journal Article
- Keywords:
Sleep deprivation;
MicroRNA-132;
MicroRNA-134
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2012;21(6):515-517
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effects of sleep deprivation on expressions of Mir-132,mir-134 in the different regions of rat brain.MethodsAll the male SD rats were divided into control group ( normal sleep group),sleep deprivation (SD).The modified multiple platform method (MMPM) was used to establish sleep deprivation model.Mir-132,mir-134 level was detected by real time PCR.ResultsMir-132 were significantly increased in SD groups in hippocampus compared with the control groups ( 51.87 ± 8.13 vs 67.25 ± 7.59 ) (P ＜0.01 ).Mir-134 were significantly decreased in SD groups compared with the control groups( 1.82 ±0.15 vs 1.45± 0.12 )(P ＜ 0.01 ).There were no statistically significant differences in cortex and thalamus (P ＞ 0.05 ).Cortex mir-132 level in SD group and control group was 1.57 ±0.10,1.48 ±0.11 respectively,and it was 1.37 ±0.09,1.36 ±0.11 in thalamus;Cortex mir-134 level in SD group and control group was 98.26 ± 5.17,100.80 ±4.15respectively,and it was 97.56 ± 6.28,91.01 ± 4.07 in thalamus.ConclusionThe upregulation of mir-132 and downregulation of mir-134 implies that two miRNAs did opposite actions in the processes of sleep deprivation.This findings indicate that hippocampus mir-132,mir-134 levels in the SD rat may reflect associated depressive patho-physiological processes.
