Evaluation of the ABX Pentra DX 120 for the Detection of Immature Cells in Peripheral Blood.
- Author:
Young Kyung LEE
1
;
Hee Jung KANG
;
Young Min KIM
;
Chul Yeon WON
;
Hyoun Chan CHO
Author Information
1. Department of Laboratory Medicine, Hallym University College of Medicine, Chuncheon, Korea. lyoungk@hallym.or.kr
- Publication Type:Original Article
- Keywords:
ABX Pentra DX 120;
Differential count;
Immature cell;
Erythroblast
- MeSH:
Basophils;
Eosinophils;
Erythroblasts;
Fluorescence;
Granulocytes;
Leukocytes;
Lymphocytes;
Monocytes;
Neutrophils
- From:Journal of Laboratory Medicine and Quality Assurance
2008;30(2):249-258
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
INTRODUCTION: The ABX Pentra DX 120 (Pentra DX 120, ABX Diagnostics, Montpellier, France) adopted new technologies to perform differential leukocyte and erythroblast counts. The double matrix can discriminate Large Immature Cell (LIC), Immature Granulocyte (IMG), Immature Monocyte (IMM), Immature Lymphocyte (IML), and Atypical lymphocyte (ALY) in addition to a routine 5-differential count. For erythroblast (ERB), a fluorescence method is employed. In this study, we evaluated the performance of the Pentra DX 120 in the performance of differential leukocyte and erythroblast counts. METHODS: Precision was evaluated using 3-level control materials. Comparison analysis was performed on 200 samples: 100 normal and 100 abnormal samples. We evaluated the 5 part differential count, LIC, IMG, IMM, IML, ALY, and ERB. These parameters were analyzed in comparison with the results from the reference method, manual differential count. RESULTS: The coefficients of variation (CVs) of precision were <5% for neutrophils and lymphocytes, <15% for eosinophils and basophils and <7% for erythroblasts. The correlation coefficients (r) were >0.9 except monocytes and basophils. IMG, ALY and erythroblasts were also well correlated with manual count (r=0.8315, 0.5602, 0.8144, respectively). The efficiency of flagging system was 84% for LIC, 80% for ALY, and 78.0% for increased ERB (>2/100WBCs). CONCLUSIONS: The Pentra DX 120 performed reliable differential leukocyte and IMG, ALY, and ERB results demonstrated comparable performance to manual count. And, the flagging system was efficient for detecting each abnormal cell population. We expect the Pentra DX 120 double matrix and erythroblast count can reduce microscopic review rate in routine laboratory and promote laboratory efficiency.
