Effect of transplantation of bone marrow mesenchymal stem cells genetically modified with human hepatocyte growth factor gene on angiogenesis in rat lung
10.3760/cma.j.issn.0254-1416.2012.04.004
- VernacularTitle:肝细胞生长因子基因修饰骨髓间质干细胞移植对大鼠肺内血管生成的影响
- Author:
Lihua LEI
;
Qun LIN
;
Caizhu LIN
;
Huizhe ZHENG
;
Xianzhong LIN
;
Fuqiu LIANG
;
Hongda CAI
;
Qing YANG
;
Youguang GAO
- Publication Type:Journal Article
- Keywords:
Hepatocyte growth factor;
Mesenchymal stem cell transplantation;
Bone marrow transplantation;
Genes;
Neovascularization,physiologic;
Lung
- From:
Chinese Journal of Anesthesiology
2012;32(4):407-410
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of transplantation of bone marrow mesenchymal stem cells (MSCs) genetically modified with human hepatocyte growth factor gene (hHGF) on angiogenesis in the rat lung.Methods Twenty F344 rats,aged 2 months,weighing 200-250 g,were randomly divided into 2 groups ( n =10 each):HGF group and control group (group C).MSCs genetically modified with hHGF was injected through the external jugular vein in group HGF.While the equal volume of DMEM culture medium (1 ml) was given instead in group C.The mean pulmonary artery pressure was detected at 28 days after transplantation.Then the rats were sacrificed and the lungs were removed for determination of the content of hHGF,expression of proliferating cell nuclear antigen (to reflect the degree of endothelial cell proliferation showed by the small pulmonary vessels) and Ⅷ factor (to reflect the density of the small pulmonary vessels),and microscopic examination.Results Compared with group C,no significant change was found in mean pulmonary artery pressure ( P ＞ 0.05),while the content of hHGF,degree of endothelial cell proliferation,and density of the small pulmonary vessels were significantly increased in group HGF ( P ＜ 0.01).No change was found in the structure of the small pulmonary vessels in group HGF.Conclusion Transplantation of MSCs genetically modified with hHGF can promote angiogenesis in the rat lung.
