Effect of lidocaine pretreatment on renal HMGB1 expression during renal ischemia-reperfusion in rats
10.3760/cma.j.issn.0254-1416.2012.04.030
- VernacularTitle:利多卡因预先给药对大鼠肾脏缺血再灌注时肾组织HMGB1表达的影响
- Author:
Xiaobing ZHU
;
Zhilong LIU
;
Zhiqun LIU
;
Lun WU
;
Yisa SHI
;
Xiyang ZHANG
;
Yi WEI
- Publication Type:Journal Article
- Keywords:
Lidocaine;
Reperfusion injury;
Kidney;
HMGB1 protein
- From:
Chinese Journal of Anesthesiology
2012;32(4):497-500
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of lidocaine pretreatment on renal HMGB1 expression during renal ischemia-reperfusion (I/R) in rats.Methods Thirty-six male Wistar rats weighing 300-350 g were randomly divided into 3 groups ( n=12 each):sham operation group (group S),I/R group and lidocaine pretreatment group (group L).Renal I/R was induced by occlusion of bilateral renal arteries for 60 min followed by 4 or 24 h reperfusion.Lidocaine 5 mg/kg was injected iv at 60 min prior to ischemia followed by 2 mg· kg- 1· h- 1 infusion iv for 60 min in group L.Equal volume of normal saline was given in group I/R.Six rats in each group were sacrificed at 4 or 24 h of reperfusionand their kidneys were removed for microscopic examination and for determination of SOD activity,MDA content and the expression of HMGB1 mRNA and protein.Results Compared with group S,renal HMGB1 mRNA and protein expression,MDA content were significantly increased,while SOD activity were significantly decreased in groups I/R and L( P ＜ 0.05).Compared with group I/R,renal HMGB1 mRNA and protein expression,and MDA content were significantly decreased,while SOD activity were significantly increased in group L ( P ＜ 0.05 ).Conclusion Lidocaine pretreatment can attenuate renal I/R injury in rats by down-regulating HMGB1 expression
