Effects of CaMK Ⅱ inhibitor KN93 on remifentanil-induced hyperalgesia
10.3760/cma.j.issn.1674-6554.2012.07.018
- VernacularTitle:鞘内注射钙调蛋白依赖性蛋白激酶Ⅱ抑制剂KN93对瑞芬太尼痛觉过敏大鼠痛行为的影响
- Author:
Chongxue CHENG
;
Jing ZHANG
;
Zhengliang MA
;
Xiaoping GU
- Publication Type:Journal Article
- Keywords:
Ca2+-calmodulin-dependent protein kinase Ⅱ;
Inhibitor,KN93;
Remifentanil;
Incisional pain
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2012;21(7):627-629
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the effects of intrathecal injection of CaMK Ⅱ inhibitor KN93 on the hyperalgesia induced by remifentanil in a incision pain model.MethodsSixty SD rats were divided randomly into 5 groups ( n =12):C group (control) ; (I) group ( incision pain ) ; R group ( incision pain + remifentanil ) ; DMSO group ( incision pain + remifentanil + DMSO) and KN93 group ( incision pain + remifentanil).In group R,DMSO and KN93,remifentanil (0.04 mg/kg,1 mg remifentanil was dissolved in 40 ml NS ) needed to be infused subcutaneously 30 min at the moment of surgery.Group DMSO and group KN93 were respectively intrathecal injected 20 μl DMSO( 10％ ) and 20 μl KN93 (50 μg/20 μl,dissolved in 10％ DMSO).Each rat received tests of the paw mechanical withdrawal threshold (PMWT) and the paw withdrawal thermal latency (PWTL) at the times of 24 h before and 2h,6h,24h,48h after surgery.ResultsCompared with the group C,the PWTL( ( 11.24 ± 0.69) s,(10.36 ±0.29)s,(11.29 ±1.12)s,(12.21 ±0.75)s) and PMWT( (25.5 ± 1.20)s,(24.92 ± 1.98)s,(25.47± 1.54 ) s,( 27.14 ± 1.04 ) s) of the group I were significantly decreased after surgery (P ＜ 0.05 ).Compared with the group Ⅰ,the PWTL( (8.48 ±0.72)s,(8.58 ±0.45)s,(8.46 ±0.92)s,(9.07±0.79) s and PMWT( (21.2± 2.42 ) s,( 19.58 ± 1.12 ) s,( 21.87 ± 1.56 ) s,( 22.26 ± 1.64 ) s ) of the group R were significantly decreased after surgery (P ＜ 0.05 ).Compared with the group R,the PWTL( ( 13.32 ± 0.73 ) s,( 11.79 ± 0.32) s,( 11.86 ±0.98)s,(12.76 ±0.82)s) and PMWT((29.75 ±1.38)s,(28.27± 1.16)s,(26.5 ± 1.02)s,(27.79 ± 1.22)s) of the group KN93 were significantly increased (P ＜ 0.05 ).ConclusionIntrathecal injection KN93 could relieve the hyperalgesia induced by remifentanil
